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Each brain hemisphere was treated separately for the NMR analysis and for RNA-seq analysis, without pooling samples, and each dataset was treated as a biological replicate

Considering that animal foods are typically the most effective sources of vitamin B12, those who follow a vegetarian/vegan diet regimen and dont consume any animal products are also most likely to have a deficiency

Because topical research uses concentration-based dosing rather than absolute-mass dosing, the researcher must formulate the peptide into a carrier base at a target concentration, then apply a consistent volume to the research area