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3.2 NTHi acquisition of host-derived haem-iron sources Despite the inherently low availability of haem-iron in the respiratory tract, NTHi is adept at scavenging a variety of host-derived haem-iron sources from intracellular and extracellular reservoirs, using a highly complex and redundant repertoire of haem acquisition systems ( Table 1 )

Lysates of Raw264.7 cells were prepared with lysis buffer containing 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 10% glycerol, 150 mM NaCl, 1% NP-40, and protease/phosphatase inhibitors (Thermo Fisher Scientific) and incubated with the peptide-conjugate resins for 1 h at 4C