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Psod-3 ::GFP reporter strain expression and heat shock experiments Approximately 200 L1 larvae from Psod-3 :: gfp synchronized animals were seeded on 60 mm plates of NGM media supplemented with 0, 20, 40, 80 or 100 mM glucose and incubated at 20C until they reached L4 larval stage

Single-cell suspensions were then loaded onto microfluidic devices, and single-cell RNA sequencing (scRNA-seq) libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with unique molecular identifier (UMI)), reverse transcription of mRNA into complementary DNA (cDNA) and amplification, cDNA fragmentation, and finally cDNA fragment cloning

Intravenous Glutathione: Dosing Differs by Route Oral glutathione absorption is notoriously poor, with most studies showing only 515% bioavailability due to rapid degradation in the gastrointestinal tract