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Furthermore, Nano-HBOCs have broad application prospects (Table 2), and have attracted increasing attention from the medical, material, chemical, biological, and engineering research attention

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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

Anamnesis, demographic and lifestyle information Including age, sex, whether the patient lives alone or with others, whether a vegan diet is followed, and the use of alcohol (g/week)