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Immunoblotting Following the indicated treatment, cell lysates were generated in ice cold RIPA lysis buffer (20 mM Tris-HCl (VWR, 97061-258), pH 7.5, 150 mM NaCl (Fisher Scientific, S271), 1 mM EGTA, 1 mM EDTA [Sigma-Aldrich, E5134], 1% sodium deoxycholate [Sigma-Aldrich, D6750], 1% NP-40 [Sigma-Aldrich, 74385]) supplemented with protease inhibitors (Fisher Scientific, PIA32955)

In conclusion, the present study found that DIS attenuated the inflammatory response and renal interstitial fibrosis and that the mechanism may be the suppression of NF-B pathway-mediated inflammatory response

Finally, we found this in vivo dietary paradigm improves therapeutic response to RSL3 and survival in an orthotopic syngeneic murine model of glioma and alters the lipid composition of tumors to create a pro-ferroptotic environment