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N Engl J Med 335(16):11821189 Olivetti G, Abbi R, Quaini F, Kajstura J, Cheng W, Nitahara JA, Quaini E, Di Loreto C, Beltrami CA, Krajewski S, Reed JC, Anversa P (1997) Apoptosis in the failing human heart

published in 2010 provides tremendous insight into the early steps in the misfolding of IAPP that eventually leads to -cell destruction

However, even though the body makes its own supply, factors like age, environmental stressors, and poor nutrition can cause those levels to dip over time

Transfer the supernatant to a new tube and add double-deionized H2O (ddH2O) to reduce the SSA concentration to 0.5% for the assay