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One example, SQSTM1 /p62, a cargo-specific receptor that eliminates ubiquitylated protein aggregates and organelles via autophagy, is a known NRF2 target gene (41,42)

Oncotarget 8:1599616016 Dwayne T, Melisa A, Fabian M, Kurt V, Lennox A-J, Donovan Mc G (2019) Dietary antioxidants in the chemoprevention of prostate cancer

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For the index PCR, a 25 L reaction containing amplicon PCR product, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer (Thermo Fisher Scientific), and 0.5 M each of Nextera XT Index Kit v2 index primers (Illumina) was prepared