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Readouts should reflect this compartment logic, combining peroxisome-targeted H 2 O 2 reporters (e.g., HyPer/roGFP-Orp1 with PTS1 SKL tagging) to track local oxidant dynamics with LCMS lipid-peroxidation markers (F 2 -isoprostanes, oxidized phospholipids, HNE adducts) to quantify downstream burden and confirm that interventions reduce peroxide leakage while preserving necessary peroxisomal metabolism (Arnaud et al

The medium was replaced with conditioned medium from BV2 cultures for 24 h

However, the excitement of these biomedical applications of AuNPs should unequivocally be balanced with testing and validation of their safety in living systems before any clinical applications

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