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Western blot analysis and immunoprecipitation Proteins from the cell conditioned media of THP-1 cells were precipitated by the addition of 3 volumes of ice-cold acetone, incubation at 20 C for 18 hours and centrifugation at 13,000 g for 10 min at 4 C

Although pharmacological treatments exist, there is a growing interest in complementary therapies to improve glycaemic control and attenuate disease progression

The 3ml size offers greater flexibility than smaller vials while avoiding unnecessary excess, making it suitable for moderate-duration protocols or multiple reconstitutions

Its well-documented physicochemical properties facilitate its use in controlled laboratory experiments without implying any clinical or therapeutic application