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Other nutrients like: Folic acid , essential for DNA synthesis and repair

2.18 Statistical analysis All experiments were conducted in triplicates or quadruplicate across three separate days, each with technical duplicates or triplicates

This multi-axis neuroendocrine regulatory profile positions DSIP as a broad hypothalamic-pituitary axis modulator, not merely a sleep-specific peptide, and makes it a research tool for studying the neuroendocrine consequences of altered sleep homeostasis and circadian rhythm disruption

DSIP was identified through its apparent ability to promote delta-wave (slow-wave) electroencephalographic activity in animal models