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doi:10.1007/s12603-013-0023-x Saruhan E, Sertoglu E, Unal Y, Bek S, Kutlu G

To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes

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As weve noted before, the function is dependent on structure, and the string of amino acids must fold into a specific 3-D shape, or conformation, in order to make a functional protein