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Methods Ethics statement All animal-based experiments were performed using the protocol (M005916-R01-A01) approved by the Animal Use and Care Committee of the University of WisconsinMadison and consistent with the standards of the National Institutes of Health

Although the Food and Drug Administration (FDA) established the mandatory fortification of all enriched cereal grain products with folic acid in 1996, full implementation, providing 140 g per 100 g of product, was achieved in 1998 (65)