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After final alkylation, all samples were diluted (1:1) with 20% ACN/0.1% FA containing 50 nM of deuterated internal standards and analyzed by LC-MS

The formation of Tyr-NO 2 can be described by the following reactions (Souza et al

Melgar M, Abrams JY, Godfred-Cato S, Shah AB, Garg A, Strunk A, Narasimhan M, Koptyev J, Norden A, Musheyev D, Rashid F, Tannenbaum R, Estrada-Y-Martin RM, Patel B, Karanth S, Achenbach CJ, Hall GT, Hockney SM, Caputo M, Abbo LM, Beauchamps L, Morris S, Cifuentes RO, de St Maurice A, Bell DS, Prabaker KK, Sanz Vidorreta FJ, Bryant E, Cohen DK, Mohan R , Libby CP, SooHoo S, Domingo TJ, Campbell AP, Belay ED

( i ) Confocal images of mitochondria (imaged by TOM20 staining) and mtDNA in cells treated with 5FU for 72 h