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HMEC1-FcRn cells (previously generated in collaboration with Novozymes) 18 were cultured in MCDB131 medium (Thermo Fisher Scientific, #10372019) supplemented with 10% FBS, 2 mM L-glutamine, 10 ng ml 1 human EGF (Peprotech), 1 ng ml 1 fibroblast growth factor (Peprotech), 50 g ml 1 gentamicin (Sigma, #G1397) and 0.25 g ml 1 Fungizone (FisherScientific, #15290018)

Indeed, the astrocyte activation and the glial scar formation were inhibited in a spinal cord injury (SCI) animal model transplanted with mesenchymal stem cells (MSCs) over-expressing HGF (Jeong et al., 2012)

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