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When using liposomes containing cationic lipids, the dried lipid film is resuspended in an appropriate solution such as sterile water or an isotonic buffer solution such as 10 mM Tris/NaCl, sonicated, and then the preformed liposomes are mixed directly with the DNA

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After the solution had entered the column, 550 l of 100 mM HEPES p H 7.0 were loaded on the column and 300 l of 100 mM HEPES p H 7.0 were used to elute the protein