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2.8 Total glutathione measurement in PBMC Quantitative determination of total glutathione levels (GSH + GSSG) in PBMC was performed using an enzymatic assay ( 2.9 ELISA assays The concentrations of free 8-hydroxy-2' -deoxyguanosine (8-OHdG) in plasma was measured using competitive assay (Stressmarq Biosciences, Canada

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GSMe is usually a much weaker inhibitor in the HEDS assay or in assays with GSSR substrates 11,14,17 , suggesting that the competition between GSH and GSMe in the roGFP2 measurements occurred at the 1st and not the 2nd glutathione interaction site

Numerous preclinical studies have also suggested that tocotrienols might be beneficial in the prevention of chronic diseases (12)