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Table 1 Plasma and Liver Biochemical Parameters Parameters SED CTRL GLN+ALA DIP Plasma parameters L-Glutamine (mmol/L) 0.82 0.06 0.79 0.02 1.26 0.031.28 0.03 L-Glutamate (mmol/L) 0.39 0.03 0.36 0.04 0.38 0.03 0.41 0.02 L-Glutamine/L-Glutamate 2.11 0.04 2.19 0.06 3.31 0.073.12 0.06 Ammonium (mol/L) 1.84 0.12 5.30 0.27* 4.1 0.183.8 0.09 Liver parameters L-Glutamine (mol/g fresh tissue) 4.31 0.23 4.17 0.20 6.23 0.556.63 0.11 L-Glutamate (mol/g fresh tissue) 1.92 0.04 2.06 0.03 2.12 0.02 2.16 0.02 L-Glutamine/L-Glutamate 2.20 0.09 2.03 0.06 2.76 0.233.04 0.05 L-Glutamine (nmol/mg protein) 26.34 1.47 24.73 0.98 33.40 1.8835.51 2.01 L-Glutamate (nmol/mg protein) 11.98 0.60 12.19 0.54 12.20 0.97 11.67 0.66 L-Glutamine/L-Glutamate 2.13 0.08 2.03 0.06 2.73 0.233.04 0.05 GSH (mol/g fresh tissue) 1.42 0.05 0.37 0.04* 0.70 0.060.69 0.08 GSSG (mol/g fresh tissue) 0.07 0.02 0.08 0.03 0.11 0.01 0.09 0.01 GSSG/GSH ratio 0.05 0.02 0.24 0.04* 0.15 0.020.13 0.02 Note

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Protocol planning Peptide cycling requires planning reconstitution timing to align with protocol phases