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Other possible signs and symptoms Hyperactivity Increased heart rate Swelling of the throat goitre Anxiety Excessive panting, heat intolerance Increased thirst Increased urination Vomiting Diarrhoea Increased volume in stool Irritability or aggression Skin lesions, dry, greasy, matted coat Heart murmurs Congestive heart failure Cardiomegaly (enlarged heart) Dyspnoea (shortness of breath) The checklist for thyroid disease A fresh and unadulterated fresh food diet Adrenal, liver, digestive immune and thyroid support Omega fats and antioxidants Vitamin deficiency test Diet for thyroid disease Hyperthyroidism has been linked to pet foods (raw commercially prepared) that include the thyroid glands of slaughtered animals

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Possible solution B: Change tissue source: use a tissue that is known to express the protein of interest, or screen several tissues and analyze simultaneously by SDS-PAGE, to compare binding proteins from different tissues