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Transfer the supernatant to a new tube, and add ddH 2 O to reduce the concentration of SSA from 0.5 to 1%

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Xc is inhibited directly by erastin or its more potent analogs imidazole ketone erastin (IKE) and piperazine erastin (PE), or indirectly by sorafenib or high extracellular glutamate concentrations.7,23 However, some cells use the transsulfuration pathway to synthesize cysteine becoming resistant to Xc inhibitors.24 Moreover, buthionine-(S, R)-sulfoximine (BSO), an inhibitor glutamate-cysteine ligase, interferes with GSH synthesis and can also promote ferroptosis.25 Molecular mechanisms of ferroptosis