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Description

Transfected COS7 cells were washed and lysed in lysis buffer (1% Triton X-100, 20 mM Tris-HCl [Ph 8], 130 mM NaCl, 10 mM NaF, 1% aprotonin, 10 g/ml leupeptin, 1 mM dithiothreitol (43816, DTT), 0.1 mM Na 3 VO 4 and 1 mM phenylmethylsulfonyl fluoride (PMSF))

DEM, drug-metabolizing enzyme