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Description

To determine albumin secretion, supernatant from 24 h was collected and the amount of albumin was determined using a human-specific albumin ELISA kit (Assay Pro) following the manufacturers instructions on an ELISA plate reader (Tecan Spark 20M)

After the fixation step in 2.5% v /v PFA for 5 min at room temperature, samples were analyzed with a Guava EasyCyte flow cytometer (Millipore) equipped with the InCyte software (Millipore)