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Chipperfield, M., Maidek, M., Pearson, P., Ashburner, M., Glover, D.M., Saunders, R.D., Duncan, I., Hartl, D., Merriam J., Lee, G., Johnsen, J., Kafatos, F.C., Siden-Kigmos, I., Louis, C., Savakis, C., "Genome maps 1991 (wall chart)", Science 254: 247-262 (1991)

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a wecA deletion) flanked by 1000 bp homology arms was prepared by Gibson assembly, and delivered as a suicide vector into wild E