lipid analysis c elegans Quick Oil Red O, Paint3D and Biopython as an economical tool to measure total levels in Caenorhabditis Lipid droplets as ubiquitous fat

In low doses, caffeic acid, when combined with caffeine, exhibits anxiolytic, antidepressant, and anti-inflammatory effects by reducing LPS-induced depressive-like behavior and decreasing TNF-alpha and IL-6 levels [34]

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Levels below 200 pg/mL indicate a deficiency in vitamin B12 while levels above 900 pg/mL may suggest excess intake or other underlying conditions

Moreover, we selected 5 mg/kg LicoA for the subsequent verification after the preliminary experiments, this dosage selection aligns with findings reported in a previous study ( Renal tubular epithelial cell (RTEC) injury and death are central to the pathogenesis and progression of CKD (Zhao et al., 2023)