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Make sure to insert the needle at a slight angle (either the 10 oclock or 2 oclock position, as shown in Figure 3)

To the best of our knowledge, the assays where DTNB, GSSG, Trx, and NADPH (at saturating concentrations) are utilized as substrates are the only assays used to measure activity of TGR and related enzymes, including human TrxR and GR 59