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Cell suspensions were lysed by freeze-thawing, and PV VLPs were purified from clarified supernatants by concentration through a 30% sucrose cushion followed by a 1545% sucrose gradient in 1 Dulbeccos phosphate-buffered saline (DPBS, Gibco), 20 mM EDTA, pH 7.0 (DPBS-EDTA) 15

Vascular edema (accumulation of extracellular water due to increased blood-brain barrier permeability) and cytotoxic edema (disruption of Na+/K + pump function on the cell membrane) are considered the underlying mechanisms of HACE ( The pathophysiology of high altitude illnesses encompasses limited oxygen supply, hypoxia-induced physiological adaptations and compensatory responses, changes in blood rheology, and the development of related tissue damage and complications