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To complete the synthesis of di- tert -butyl ester of (2 R ,3 S )- 2 compound 16 was first transformed into the ester and later deprotected to the diol 17 which was selectively oxidized, again esterified and finally the phenylsulfonyl group was removed electrochemically (Scheme 4) [52]

They found that two commercially available beetroot juices inhibited in vitro radical formation in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and (3-ethylbenzothiazoline-6-sulfonicacid) ABTS assays by 100% and 92%, respectively

This, in turn, reduces inflammatory cell infiltration and alleviates colon injury

FXR agonists can also influence the gut microbiota composition