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Briefly, cerebellar parasagittal slices (350 m thick) were cut on a McIlwain tissue chopper and transferred onto 30 mm diameter Millipore culture inserts with 0,4 m pores (Millicell, Millipore, PIHP03050)

Mechanical Agitation Damage Shaking a peptide vial is the single most common way researchers unintentionally damage a compound during reconstitution

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Regular metabolic panels include fasting glucose, lipids, and liver enzymes to track metabolic effects