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Regulatory bodies advocate for the use of multivariable statistical approaches, particularly at Stage 3 of CPV, to ensure correct batch production and reproducibility by implementing non-invasive sensors during production, known as Process Analytical Technologies (PAT) (Read et al., 2010)

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Temel, Y., Kfreviolu,

Enrichment of labeled proteins by ITalk To validate novel itaconated proteins, Raw264.7 cells were grown to 80% confluence and treated with DMSO or 1 mM ITalk for 2 hours