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The membranes were treated with 5% non-fat milk for 1 h and incubated with the primary antibodies raised in the mouse/rabbit against DDDDK (Flag) (Abcam, ab205606), HA (Abcam, ab9110), CARNMT1 (CNMT) (Atlas Antibodies, HPA02675), TFR1 (Abcam, ab214039), TF (Abcam, ab277635), Fth1 (Abcam, ab183781), pan-Met-His (Bioworld, BS74112), SOD1 (Abcam, ab308181), actin protein (Abclonal, AC026), and beta-Tublin (Abcam, ab314069) overnight, followed by washing in tris-buffered saline (TBST)

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Common additions include: Ipamorelin: A growth hormone secretagogue that supports fat metabolism, muscle preservation, and additional collagen stimulation through increased GH levels Ipamorelin with semaglutide BPC-157: A body protection compound that supports gut health and tissue healing, which can complement GHK-Cu's repair functions Vitamin C supplementation: A cofactor required for collagen synthesis that amplifies GHK-Cu's collagen-building effects Each addition should be discussed with your physician and introduced one at a time to monitor tolerance and response

From those, 32 transcripts had a full length ORF with an average of 1,200 bp