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DNA hairpin for magnetic tweezers experiments The design of a DNA hairpin substrate consisting of a 1238 bp hairpin with a 4 nt loop (4 dTs), a 74 bp dsDNA fragment ligated to a 126 bp highly biotinylated dsDNA handle, and a 146 bp 3-digoxigenin labelled dsDNA tail was based on a previous published construct with some modifications 79

While certain NF-B-regulated genes play a major role in regulating the amount of ROS in the cell, ROS have various inhibitory or stimulatory roles in NF-B signaling

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Further studies have however shown that genetic aberrations present in hiPSC clones are not de novo mutations generated during the reprogramming process, but are most likely derived from the original somatic cells from which the hiPSC were reprogrammed 41