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2021;13(7) doi: 10.3390/pharmaceutics13071003

Gut microbiota inoculum preparation from human feces for ex vivo study One gram of raw fecal sample was mixed with 10 mL of PBS supplement with 0.05% -cysteine in a tube containing glass beads, homogenized the mixture by vortexing for 1 min in an anaerobic chamber (Whitley DG250 Workstation, Don Whitley Scientific Limited, UK), filtered the homogenized fecal liquid through a Falcon 100 m cell strainer, and subsequently used the fecal filtrate for the study

This may be especially true if you drink dark liquors, like bourbon and brandy

Scenario 3: Post-bariatric surgery patient adding semaglutide A 52-year-old had gastric bypass 5 years ago