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Prolactin activates tyrosyl phosphorylation of insulin receptor substrate 1 and phosphatidylinositol-3-OH kinase

Electrophoretic Mobility Shift Assay (EMSA) 5Cy5-DNA probes were produced commercially and 3-UTP-Cy5 ligation was performed at 37C on double-strand DNA with terminal Deoxynucleotidyl Transferase (TdT) (Promega)