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Primary antibodies were detected with anti-rabbit HRP-conjugated polymer and 3,3-diaminobenzidine substrate, followed by counterstaining with haematoxylin, all of which were provided in the Leica BOND Polymer Refine Detection System (DS9800)

Antitumor efficacy evaluations demonstrated that these immunoliposomes maintained the potent therapeutic effect of IL-2 while reducing systemic toxicity across multiple tumor models

They found that in primary cortical neurons, doxorubicin treatment markedly reduced the expression of breast cancer type 1 susceptibility protein which is the key factor for DNA repair (Manchon et al., 2016)