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doi: 10.1016/j.cell.2017.09.021 3 TangDChenXKangRKroemerG

For signal detection, the ABI Prism 7700 sequence detector (Life Technologies, CA, USA) was programmed to execute an initial step of 2 min at 50C and 10 min at 95C, followed by 40 thermal cycles of 15 sec at 95C and 1 min at 60C