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The conditions of real-time PCR were as follows: (1) initial PCR activation 95 C, 10 min, (2) 3-step cycling for 35 cycles involving (a) denaturation 95 C, 30 s, (b) annealing 58 C, 1 min and (c) extension 72 C, 1 min, followed by a dissociation phase

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Cell Death Dis 16(1):99 Lee J, Roh J-L (2025) Ferroptosis: iron release mechanisms in the bioenergetic process

A small percentage of Hb is always auto-oxidized under normal physiological conditions resulting in the presence of metHb in blood, but metHb can also be present due to genetic abnormalities, exposure of red cells to certain drugs or toxins, or due to a deficit in antioxidants