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Chemiluminescence was measured immediately at 415 nm using a microplate reader (SpectraMax i3x, San Jose, CA, USA)

Continued use is recommended to maintain beneficial effects

In the first case, we first numerically solve the subsystem eq(6) and eq(7) for t = t * separately under the assumption H = H max with initial condition C 0 = 1 and for a range of one to seven days to obtain the value of the CYP enzyme levels of C for different amount of alcohol intake ( E 0 )