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Luminescence was measured using a ViewLux plate reader (Perkin Elmer), with data reported as relative luminescence units

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Additionally, another study confirmed that CU significantly inhibited the overexpression of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-), reduced lipid peroxidation, and restored MDA levels, which collectively ameliorated neurological deficits, alleviated cerebral vasospasm, and significantly decreased mortality in SAH (Luo et al., 2011)

It is important to note that melatonin modulates the immune system by suppressing proinflammatory molecules in a dose-dependent fashion due to its physiological circadian pattern