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& An inventory for measuring depression

Generation and validation of Atf4 -/- and reconstituted cell lines -/- Tsc2 +/+ (WT) and Tsc2 -/- MEFs lacking Atf4 were generated by CRISPR-Cas9 mediated deletion using pSpCas9n(BB)-2A-GFP (PX461) vector (Addgene, 48140) according to the previously described protocol (Ran et al., 2013)