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A 2 L aliquot of each sample was injected into a Orbitrap Fusion Lumos (Thermo Fisher Scientific, Waltham, MA) or Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific) coupled to an UltiMate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific)

The STEP 1 trial, published in the New England Journal of Medicine, demonstrated 14.9 percent mean body weight reduction with the 2.4mg weekly dose over 68 weeks, compared to just 2.4 percent with placebo