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The covariate approach was applied according to Slob and Setzer (2014) with the assumption that the maximum response (parameter c) and the log-stepness (parameter d) are constant across different datasets

Furthermore, buying experimental substances online is dangerous because products can be contaminated or contain completely different doses than what is listed on the label

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Total RNA was extracted from the cell lysates using an EZ1 RNA Cell Mini Kit (Qiagen GmbH) on EZ1 Advanced XL automated RNA purification instrument (Qiagen GmbH) following the manufacturer's instruction