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Make sure you are using a 1 mL (100-unit) syringe

The single-cell quantitative RTPCR (qRTPCR) was performed at the Federal Institute of Technology (ETH) Zurich, Department of Biosystems Science and Engineering (D-BSSE) and Genomics Facility in Basel by using C1 Single-Cell Autoprep and BioMark HD instruments (Fludigim, San Francisco, CA)