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Moreover, in rat brain slices both GABA and glutamate uptake and release rates show very rapid and pronounced quantitative fluctuations during early development (Schousboe et al., Metabolism of GABA along the pathway suggested in Figure 7 would eliminate a need for uptake of exogenous aspartate and its conversion to malate in astrocytes in an aspartate-dependent pathway model for GABA formation suggested by LaNoue et al

For protein quantitation, reverse and contaminant proteins were removed