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The formulation of nanobiosilica in an AGS seed coating resulted in the alleviation of salt stress on mung seedlings by increasing the activity of enzymes involved in the plant defense system, decreasing the level of reactive oxygen species and lipid peroxides, compensating the necessary l-proline content, and increasing the photosynthetic pigments and NO content, as well as the specific extracellular H + level into the medium, probably by re-establishing the equilibria of membrane transporter activities