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Protect from light and moisture, keep sealed until reconstitution in a laboratory setting

doi: 10.3389/fenvs.2015.00025 Received 18 January 2015 Accepted 13 March 2015 Published 31 March 2015 Volume 3 - 2015 Edited by Naser A

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In reaction mixture without enzyme solution, varying amounts of glutathione (0-300 l of stock solution giving 0-260 M final glutathione concentrations) were reacted with 150 l of monobromobimane solution (final concentration 50 M) in presence of phosphate buffer (3.0 ml) in cuvettes