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The system was trained with a series of standard solutions of cyclic-di-GMP and the limit of detection limit was determined

Collectively, these observations indicate that, despite their similar BMI, obese subjects with low IGF-1 levels are markedly more predisposed to have multiple metabolic abnormalities, such as increased adiposity, inflammation, dyslipidemia and hyperuricemia than subjects in the standard IGF-1 range

The definition of vitamin B12 deficiency varies with the assay used but generally includes serum cobalamin levels below 148 pmol/L plus symptoms, or elevated serum homocysteine, or increased methylmalonic acid (MMA) (74)