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pre-determined in the same assay in the absence of competing serum) to each well containing serially diluted pooled sera from each group, followed by a 1 h incubation at 37 C

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Safety Precautions When handling GHK-Cu powder, observe the following safety measures: - Wear gloves and a face mask - Work in a clean, well-ventilated area - Avoid direct skin contact with the undiluted powder - Store the powder in a cool, dry place away from direct sunlight Step-by-Step Mixing Process Determining the Correct Concentration The optimal concentration of GHK-Cu in a solution typically ranges from 0.5% to 2%