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Our results show that GHK and GHK-PEG induce apoptosis in SH-SY5Y neuroblastoma cells at 1 and 10 nM with GHK-PEG demonstrating greater effects ( Figure 5 )

A: Preclinical studies have explored concentrations from approximately 1 nM to 10 M in vitro, with in vivo models typically using formulations calibrated to deliver peptide within a physiologically relevant range at the wound site

Since cell membranes are negatively charged, peptide drugs can efficiently penetrate membranes when their overall charge is neutral or positive 42

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