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Cell culture and transfection/electroporation 293 T human embryonic kidney cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 units/mL of penicillin, and 100 g/mL of streptomycin and then transfected with expression vectors via the calcium phosphate-DNA precipitation method 33

Although the exact mechanism of action remains to be elucidated, the main mechanism includes inhibition of cyclooxygenase and lipoxygenase, subsequently leading to reduced production of prostaglandins and leukotrienes, respectively [25]

Re-addition of NFT resulted in Nrf2 re-accumulation, GCLC and glutathione synthesis followed this trend in cells treated with 20 M NFT

Boosts Sleep Quality : By helping you feel more relaxed and less anxious, L-Theanine can make it easier to fall asleep