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2.6 Analysis of oxidative stress SOD activity, glutathione (GSH) content, and malondialdehyde (MDA) levels (all of which are indicators of the oxidative stress burden) were measured in hepatic tissue homogenates using the colorimetric kits obtained from BioVision, Milpitas, CA, United States: SOD kit (Cat: K335100), GSH kit (Cat: K464100), and MDA kit (Cat: K739100), according to the guidelines provided by the manufacturer

The eluted protein was then dialyzed against 10 mM Tris and 150 mM NaCl (pH 7.4)