glutathione glutamate release induces of from cultured rat astrocytes – a possible neuroprotective mechanism? - Frade - 2008 - Journal of Neurochemistry 5 Route of synthesis of

Amino acid transporters, specific metabolic enzymes, autophagy-associated proteins and even the amino acid itself can all be potential targets for controlling cancer growth

doi: 10.1016/j.celrep.2021.109050 45 Garcia-BermudezJBaudrierLLaKZhuXGFidelinJSviderskiyVOet al

US Patent 9,365,512 B2 Amberg W, Lange UE, Ochse M, Pohlki F, Behl B, Relo AL, Hornberger W, Hoft C, Mezler M, Sydor J (2018) Discovery of novel aminotetralines and aminochromanes as selective and competitive glycine transporter 1 (GlyT1) inhibitors

Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment